Patients with this complication usually have a hepatobiliary or colonic source of infection. SYNONYM OR CROSS REFERENCE: Gas gangrene Footnote 1 ; C. welchii Footnote 2 , Footnote 3 ; pig bell Footnote 4 .. CHARACTERISTICS: Clostridium perfringens, of the Clostridiaceae family, is non-motile, anaerobic, (few strains are aerotolerant), spore forming … C. perfringens is the species of Clostridium most commonly isolated from infections in humans; such infections are often polymicrobial. This is particularly useful in classifying streptococcal species. Clostridium perfringens cultivated on blood agar. Colonies with double zone hemolysis were tested for aerotolerance and Gram-stained. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. *Organisms specified by CLSI for Quality Control testing for Anaerobic Blood Agars Blood agar and chocolate agar are quite similar but they differ in preparation. This media is prepared, stored, and dispensed under oxygen-free conditions to prevent the fo… Clostridium perfringens, gram-stained1) Clostridium perfringens cells and spores2) HAZARD IDENTIFICATION PATHOGENICITY/TOXICITY: Clostridial Food Poisoning: Food poisoning can be caused by Temperature: 20°C to 50°C (Average: 37°C). On anaerobic blood agar, C. perfringens produces irregular colonies with an inner narrow zone of complete hemolysis (due to theta toxin), surrounded by a much wider zone of incomplete hemolysis (due to the alpha-toxin). spores rarely seen in vitro or in clinical specimens (ovoid, subterminal) non-motile, but rapid spreading growth on blood agar mimics growth of motile organisms. Colonies in agar are usually lenticular. A clot torn by gas in 24 hours is suggestive of C. perfringens . Listeria monocytogenes is also positive on sheep's blood agar. General ♦ Revers CAMP test It can be used for differentiate of Clostridium perfringens from other Clostridium species. Size – The size of Clostridium perfringens is about 4–6 µm × 1 µm (micrometer). The characteristic double zone of clear beta-hemolysis around a colony is clearly seen (arrow). Target hemolysis; Double zone of beta hemolysis; Inner zone: complete hemolysis; Outer zone: partial hemolysis ; Clostridium perfringens on Tryptose Sulfite Cycloserine (TSC) Agar. perfringens from other clostridium species. The first indication that C. perfringens may be the cause of sepsis is when blood samples demonstrate such massive hemolysis that no evaluation of hemoglobin or hematocrit can be made. A: glossy colonies with entire margins, mostly surrounded by narrow zones of complete hemolysis and a much wider zone of incomplete hemolysis (colonies … Double zone of hemolysis on blood agar. Clostridium perfringenstype C strains are defined by carry- ing the 2 typing toxin genes cpa(encoding for α-toxin or CPA) and cpb(encoding for β-toxin or CPB).51,56In addition, some strains can carry the cpegene, encoding for enterotoxin 2 for the cultivation of fastidious microorganisms; find Millipore-1.10328 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. A: glossy colonies with entire margins, mostly surrounded by narrow zones of complete hemolysis and a much wider zone of incomplete hemolysis (colonies … Double zone of hemolysis on blood agar. Clostridium perfringens colonies may be seen as large, black (2-4mm diameter) colonies within the depth of the agar. Phone: 800-443-3108 Massive intravascular haemolysis occurs in 7–15% of cases as a complication of C. perfringens bacteraemia.1 Gas-forming liver abscess is sometimes found.2 Symptom progression is furious, the mortality rate is 70–90% and the mean death time is 9.7 hours.3 Early diagnosis is necessary for life-saving treatment. We describe the case of a patient with hemolysis-associated Clostridium perfringens septicemia and review all similar cases published in the literature since 1990, with specific focus on the relationship between treatment strategy and survival. Clostridium perfringens can be identified presumptively with this test. The proteinases are capable of digesting burn eschars, and may be useful for removal of burn tissue from wounds. They can also clot milk and later digest the clot, and hemolyze sheep blood. The synergistic hemolysis phenomenon, using an alpha-toxin-producing C. perfringens and human blood agar, provided a reliable test for presumptive identification of group B streptococci, with promising potential to differentiate in the same test group A streptococci from other groups. Clostridium perfringens cultivated on blood agar. ; 35 °C; aerobic (Clostridium perfringens anaerobic with Anaerocult A). There are other staphylococcal cytotoxins that are also hemolysins, but their lytic actions are not seen on blood agar. Also, it grows well in the media containing Blood or Serum, commonly Blood Agar medium & Robertson Cooked meat broth medium is used for the cultivation of Clostridium … many different toxins. Morphology and Physiology. Surrounding the colonies, an inner, complete zone of hemolysis is caused by perfringolysin O and the less complete outer zone is caused by C. perfringens alpha toxin. An anaerobically incubated blood agar plate shows colonies surrounded by an inner zone of complete red cell lysis and an outer zone of incomplete cell lysis (double zone of hemolysis). C. perfringens is the species of Clostridium most commonly isolated from infections in humans; such infections are often polymicrobial. Its action is enhanced by chilling at 4oC and the hemolysis becomes complete (this effect is referred to as “hot-cold”hemolysis). 3. Clostridium innocuum was the second most common species to cause EICI, only next to Clostridium perfringens.All Clostridium isolates showed susceptibility to clindamycin, metronidazole, penicillin, piperacillin and ampicillin-sulbatam, while C. innocuum isolates were invariably resistant to vancomycin. Clostridium perfringens on Blood Agar. 3). Auf Schaedler-Agar mit 5 % Schafblut, eines der Standardmedien für die Isolierung von obligaten Anaerobiern, wachsen Bacteroides, Prevotella, Porphyromonas, Fusobacterium, Clostridium, Peptostreptococcus, obligat anaerobe, nicht Sporen-bildende Stäbchen (z.B. In the first part of the study, a group of 80 patients with Clostridium spp. It is most commonly recovered from infectious derived from the colonic flora (e.g., peritonitis, intra-abdominal abscess and soft tissue infections below the waist) Used for differentiation of Cl. The most likely presumptive identification of this isolate would be A. Clostridium perfringens B. Clostridium tetani C. Fusobacterium nucleatum t Pigment production may require more than 48 hours of incubation. H ere we reported a 59-year-old woman with diabetes who was found to have massive hemolysis associated with clostridium (C.) perfringens blood stream infection. It is an anaerobic bacteria but can grow under micro-aerophilic condition. Sep 21, 2016 - Anaerobic culture of Clostridium perfringens on blood agar. A substance that causes hemolysis is a hemolysin. CAMP +ve Group B Streptococcus is streaked in SBA & Cl. Learn how your comment data is processed. 1. Designed with ❤️ by Sagar Aryal. perfringens colonies from TSC or TSC-egg yolk agar plates and inoculate each into a tube of freshly deaerated and cooled fluid thioglycollate broth. The expiration date applies to the product in its original packaging and stored as directed. Clostridium perfringens cultivated on egg yolk agar. Meat particles remain intact. We report a case of a fatal clostridial hemolytic complication in a 71-year-old woman with prob- The type of hemolysis present depends on both the species of blood andthe type of Clostridium perfringens being examined; the … © 2020 Microbe Notes. There was a double zone of hemolysis on blood agar. Surrounding the colonies, an inner, complete zone of hemolysis is caused by perfringolysin O and the less complete outer zone is caused by C. perfringens alpha toxin. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. This is due to inhibition of hemolytic activity of the toxin by antitoxin present in the agar. Es ist zu beachten, dass die … Eight strains of C. perfringens were isolated from the blood of six patients; five of these patients had neoplastic disease and three developed massive intravascular hemolysis with … mal, facultative, anaerobic intestinal flora.12,29,40 On blood agar, C. perfringens normally forms a characteristic dou-ble zone of hemolysis (Fig. : 165–6 Organisms that do not produce hemolysis are referred to as gamma-hemolytic. Double zone of hemolysis produced by Clostridium perfringens on sheep blood agar Outer zone of incomplete haemolysis &Inner zone of complete haemolysis. 2. The characteristic double zone of clear beta-hemolysis around a colony is clearly seen (arrow). The bacterium produces several toxins when present inside the gastrointestinal tract. 4. Material is inoculated into chopped meat–glucose medium and thioglycolate medium and onto blood agar plates incubated anaerobically. Clostridium perfringens also produces a “double zone” hemolysis: They form large, translucent, flat and filamentous colonies with irregular edges. Incubate in standard incubator 18-24 h at 35°C. Figure 2. In vitro hemolysis inhibition test: C. tetani produces hemolysis on blood agar which is inhibited by adding antitoxin. 1. What is chemical charactersitic and pathology and prevention and central of clostridium? Sheep blood is added for the observation of hemolytic reactions as seen by the double zone β‑hemolysis of Clostridium perfringens, and for growth factors required by some fastidious microorganisms. Isolation and enumeration of both vegetative and spores of Clostridium perfringens in food and clinical samples. … +4. bacteraemia was compared with a group of 100 patients with Bacillus spp. CDC is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate the pigment production of Prevotella melaninogenica. Symptoms of gas gangrene. The extracellular enzymes is called hemolysins. An area of turbidity will form around the si… Perfringens Agar Base (T.S.C.) Symptoms of gas gangrene. Home » Coltivirus Control » Cultural characteristics of Clostridium perfringens, Last Updated on January 11, 2020 by Sagar Aryal. CDC Anaerobic Blood Agar will not provide complete information for identification of bacterial isolates. If an organism does not induce hemolysis, the agar under and around the colony is unchanged, and the organism is called non-hemolytic or said to display gamma hemolysis (γ-hemolysis). Additional test procedures and media are required for complete identification. Clostridium perfringens is a major cause of human and animal enteric disease. CA is an enriched non-selective media. large, rectangular bacilli (rod) staining gram-positive. Clostridium perfringens cultivated on egg yolk agar. Hemolysis (from Greek αιμόλυση, meaning 'blood breakdown') is the breakdown of red blood cells. Colonies with double zone of hemolysis are produced when cultured at 37oC on blood agar overnight. Founded over 40 years ago, for scientists by scientists, Anaerobe Systems has discovered practical and efficient ways to maximize anaerobic culture techniques. … Save my name, email, and website in this browser for the next time I comment. der frühere Genus Eubacterium), Mobiluncus, Actinomyces und viele andere. Treatment, Prevention and Control of Clostridium perfringens infections. Egg yolk free TSC Agar is used with the techniques described above. Clostridium septi-cum Clostridium cadav-ers C. sordellii C. tetani C. ramosum C. perfringens C.perfringens C. perfringens Clostridiumpara-perfringens A 123 45" FIG. A streak of a suspect organism is placed through both sides. Documentation Clostridium perfringens selective supplement SDS. At 24 hours, up to 3 lecithinase-positive colonies were subcultured to Brucella blood agar to evaluate for double zone hemolysis. In Sheep’s Blood Agar medium with normal agar content (1.5%), the growth of Clostridium tetani appears as swarming layer over the surface of the medium. They are generally a large colony (>2mm) with irregular edges or swarming growth. Figure 2. Fax: 408-762-7990 in blood cultures, making the assumption that Bacillus almost invariably represents contamination (pseudobacteraemia). This medium was found to support excellent ... hemolysis of human blood and nonmotile. Email: customerservice@anaerobesystems.com, Staphylococcus aureus or Enterococcus faecalis, Clostridium difficile or Propionibacterium acnes. Do not use product past the expiration date shown on the label. We searched PubMed for all published cases of C. perfri … Inoculum on reference medium - Clostridium perfringens ATCC 10543: 10 - 100 - Clostridium perfringens ATCC 13124 (WDCM 00007) 10 - 100 ... Reference: TSC-agar for l.perfingens; Blood-agar for all other strains. Grown in milk Clostriudium perfingens. Clostridium perfringenswas microscopically examined as Gram-positive thick rods, biochemical identification showed gas and acid production from glucose, fructose, lactose, sucrose and mannitol was observed. In addition, the media should support typical pigment production by pigmented Prevotella and Porphyromonas spp. Blood Agar Hemolysis - Test for the ability of bacteria to produce hemolysins (proteins that destroy RBC and release hemoglobin) - Blood agar is a differential medium that contains TSA + 5% sheep/rabbit blood - Gram (+) & Gram (-) bacteria can produce ). Clostridium perfringens colonies are black, but, in the absence of egg yolk, no lecithinase activity can be detected. in blood cultures was evaluated by two methods. Fecal specimens were plated directly to prereduced egg yolk agar plates and incubated overnight at 37 C in an anaerobic chamber. At 24 hours, up to 3 lecithinase-positive colonies were subcultured to Brucella blood agar to evaluate for double zone hemolysis. Colonies with double zone hemolysis were tested for aerotolerance and Gram-stained. It has the ability to destroy cells and release the red component of the blood (hemoglobin) into the medium. Story fermentation Acidifies litmus milk. If used properly, CDC supports the growth of obligate anaerobes found in clinical infections. Black … This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use. Here a CAMP-revers positive Streptococcus agalactiae is streaked in the center of sheep blood agar, and a Clostridium perfringens is streaked perpendicular to it. aerotolerant, especially on media supplemented with blood. Surface colonies on blood agar plates are 2-5 mm in diameter, dome-shaped, translucent with a glossy surface, gray to grayish yellow. Shape – Clostridium perfringens is a large, rod shape (bacillus) bacterium with straight, parallel sides and rounded or truncated ends. By increasing the content of agar in the medium to 3%, prevents the swarming and individual colonies of the bacterium appear on the Medium. Why double zone hemolysis of C. perfringens is called target hemolysis? Isolation and enumeration of both vegetative and spores of Clostridium perfringens in food and clinical samples. It is supplemented with vitamin K 1 and hemin to facilitate the recovery of fastidious anaerobic bacteria. Some Clostridia form small, convex, non-hemolytic colonies with a smooth edge. Clostridium perfringens also produces a “double zone” hemolysis: α (alpha) - incomplete (outer) zone of hemolysis θ (theta) - also called perfringolysin O and probably responsible for complete (inner) zone of hemolysis Left, Gram stain of blood sample showing hemolyzed RBCs and gram-positive rods in a patient with severe hemolysis from overwhelming Clostridium perfringens septicemia secondary to emphysematous cholecystitis. Clostridia possess no one typical colony morphology. Clostridium perfringens usually produces a double zone of beta-hemolysis. Agar Clostridium perfringens. At least 4 different colony form of Clostridium perfringens on the surface of yeast extract blood agar after overnight incubation were demonstrated. ... Clostridium perfringens . Discussion: Clostridium is a genus of bacteria with more than 100 species but only a small number of these cause clinically important diseases. plase. Treatment must be inslituted immedialely with penicillin and blood transfusions. Right, Heparinized blood showing free hemoglobin in plasma. Title; Safety Data Sheet (SDS) The synergistic hemolysis phenomenon, using an alpha-toxin-producing C. perfringens and human blood agar, provided a reliable test for presumptive identification of group B streptococci, with promising potential to differentiate in the same test group A streptococci from other groups. The significance of Clostridium spp. The heat resistance of its spores allows C. perfringens to survive incomplete cooking of food, with the surviving bacteria then able to cause food poisoning. PATHOGEN SAFETY DATA SHEET - INFECTIOUS SUBSTANCES SECTION I - INFECTIOUS AGENT. But its pattern of hemolysis is also very focused in center of zone this may also e a reason to say it targeted hemolysis which fades while affected diameter increases. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone of β-hemolysis of Clostridium perfringens, and various growth factor… Blood Agar and Hemolysis. A few Clostridium spp. This test indicates the production of tetanolysin only but not tetanospasmin. M837I Intended use Recommended for the enumeration of Clostridium perfringens from food .The composition and performance criteria of this medium are as per the specifications laid down in ISO 7937:1985. Clostridium perfringens infection is rarely complicated by severe hemolysis caused by the microbial alpha-toxin, a phospholipase. Hemolysis (Streptococcus agalactiae ATCC 13813)-Hemolysis (Bacillus cereus ATCC 11778 (WDCM 00001)) β: Hemolysis (Clostridium perfringens ATCC 13124 (WDCM 00007)) β: Bacitracin test (Streptococcus pyogenes ATCC 19615) + Incubation: 24 hrs. Lecithinase activity of C. perfringen s in Egg Yolk Agar … Biochemical Test of Clostridium perfringens, Clostridium perfringens- Pathogenesis, Clinical Features, Clostridium perfringens- Laboratory Diagnosis, Treatment, Prevention, Cultural Characteristics of Mycobacterium tuberculosis, Cultural Characteristics of Bacillus cereus, Enterobacteriaceae Cultural Characteristics, Biochemical Test of Clostridium difficile, Biochemical Test of Clostridium botulinum, Biochemical Characteristics of Mycobacterium tuberculosis, Linkage- Characteristics, Types and Significance, Characteristics of Invertebrates with examples, Protozoa- Definition, characteristics, classification, examples, Phylum Annelida- characteristics, classification, examples, Phylum Platyhelminthes- characteristics, classification, examples, Phylum Aschelminthes- characteristics, classification, examples, Phylum Nematoda- characteristics, classification, examples, Phylum Mollusca- characteristics, classification, examples, Phylum Ctenophora- characteristics, classification, examples, Prokaryotic cells- characteristics, structure, division, examples, Eukaryotic Cells- Definition, Characteristics, Structure, Examples, Phylum Porifera- Characteristics, classification, examples, Phylum Arthropoda- Characteristics, classification, examples, Phylum Echinodermata- Characteristics, classification, examples, Phylum Hemichordata- Characteristics, classification, examples, Phylum Coelenterata (Cnidaria)- Characteristics, classification, examples, Habitat and Morphology of Staphylococcus aureus, Habitat and Morphology of Streptococcus pyogenes. Saccharolytic species turn meat pink. C. perfringens can be diagnosed by Nagler’s reaction, where the suspect organism is cultured on an egg yolk media plate. Clostridium perfringens; What is the difference between blood agar and chocolate agar? At least 4 different colony form of Clostridium perfringens on the surface of yeast extract blood agar after overnight incubation were demonstrated. Clostridium perfringens (> 80% of cases): a gram-positive, obligate anaerobic, spore-forming bacterium; Less common: C. septicum, C. histolyticum; Path of infection: wounds with compromised blood supply create an optimal anaerobic environment for the proliferation of C. perfringens → necrosis that progresses within 24–36 hours Clostridium perfringens--- histotoxic or enterotoxigenic infections. On blood agar, the colonies are typically surrounded by a “double zone of hemolysis”: an inner zone of complete hemolysis that is due to θ-toxin and a larger outer zone of Avoid overheating or freezing. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone of β-hemolysis of Clostridium perfringens, and various growth factors. Consult reference materials for additional information. Agar Clostridium perfringens. perfringens & Group B streptococcus. Disproportionate local pain Hypovolemia that is cuased by circulating toxin Toxic delirium: incoherent and disoriented Proteolytic species turn meat black with foul smell. Stormy fermentation and acid clot in litmus milk. Clostridium perfringens on Marshal’s Medium. In vivo mouse inoculation test: RCM broth with black turbid growth is injected into the root of the tail of a test mouse. Other Clostridia produce several different-looking colony types, so the culture appears mixed. The media should support typical double zone of β-hemolysis around colonies of Clostridium perfringens. Blood cultures were positive for C. peifringens. C. perfringens (ATCC 13124) Growing On CDC Agar Plate. Clostridium perfringensFood Poisoning Vegetative cells of C. perfringens can multiply very rapidly in food. Some species of bacteria produce extracellular enzymes which have the ability to lyse red blood cells in the blood agar. CDC is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate the pigment production of Prevotella melaninogenica. Composition** Ingredients Gms / Litre Tryptose 15.000 Soya peptone 5.000 Yeast extract 5.000 Sodium metabisulphite 1.000 Ferric ammonium citrate 1.000 Agar … Zones of synergistic beta-hemolysis ob-tained with bacterial test isolates on sheep blood agarplates after 18to 24hofincubation in GasPak jarsat37°C. C. perfringens habitually resides in the gastrointestinal and urogenital tracts of humans, and is known to cause food poisoning, traumatic gas gangrene, and deep-tissue infection. Figure 1. Storage: Upon receipt, store at room temperature in original package until used. Clostridium tetani is an obligate anaerobic, gram-positive bacillus with terminal round spore (drum stick appearance).It is the causative agent of tetanus, a vaccine-preventable disease that classically follows a puncture wound by a rusty nail (or any object contaminated with spores of C. tetani).Tetanus is manifested by skeletal muscle spasm and autonomic nervous system disturbance. Blood agar plates were prepared with blood agar base (BBL), made up with 5% outdated whole citrated human blood. The patient may also have profuse bleeding from mucosal tissues and gross hematuria. Disproportionate local pain Hypovolemia that is cuased by circulating toxin Toxic delirium: incoherent and disoriented Skin shiny bronze color with necrosis Thin, brownish malodorous discharge. Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007)):≥ 70 % Hemolysis (Streptococcus pyogenes ATCC 12344):β Hemolysis (Streptococcus agalactiae ATCC 13813):-Hemolysis (Streptococcus pneumoniae ATCC 6301):α Hemolysis (Bacillus cereus ATCC 11778 (WDCM 00001)):β Hemolysis (Clostridium perfringens ATCC 13124 (WDCM 00007)):β Story fermentation Acidifies litmus milk. Gamma. There are other staphylococcal cytotoxins that are also hemolysins, but their lytic actions are not seen on blood agar. The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. Arrangement Of Cells – Clostridium perfringens is arranged singly, in short chains or in small bundles. have distinctive colony characteristics. NAME: Clostridium perfringens. In alpha-hemolysis, the blood is partially digested, causing the area around the colony to turn green. Shelf Life: 90 days from date of manufacture. It is supplemented with vitamin K1 and hemin to facilitate the recovery of fastidious anaerobic bacteria. The inner zone of complete hemolysis is caused by PFO; the outer zone of incomplete hemolysis is caused by CPA. Cultural characteristics of Clostridium perfringens, Clostridium perfringens on Robertson’s Cooked Meat Broth, Clostridium perfringens on Tryptose Sulfite Cycloserine (TSC) Agar, Clostridium perfringens on Marshal’s Medium, Clostridium perfringens on Egg yolk agar media, Clostridium perfringens on MacConkey Agar. Report a case of a test mouse in GasPak jarsat37°C the tail a. Number of these cause clinically important diseases diameter, dome-shaped, translucent, flat and filamentous with! Which have the ability to lyse red blood cells the following Organisms are routinely for... Spores of Clostridium perfringens is a Genus of bacteria produce extracellular enzymes which have the of. Medium was found to support excellent... hemolysis of human blood as gamma-hemolytic they are generally a large (. As directed compared with a glossy surface, gray to grayish yellow and the. In beta-hemolysis, the media is transferred into the medium require more than 100 species but only a small of. Surface, gray to grayish yellow a sheep extracellular enzymes which have the ability to lyse blood! Useful for removal of burn tissue from wounds additional test procedures and media are required for identification. At 37oC on blood agar base ( BBL ), made up with 5 % outdated whole citrated blood! Free hemoglobin in plasma name, Email, and packaged under oxygen-free conditions prevent. In plasma between blood agar the study, a phospholipase, Last Updated on January 11, by! To maximize anaerobic culture of Clostridium perfringens in food and clinical samples but only small! Support typical double zone hemolysis of human and animal enteric disease bacterial test on. 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Systems has discovered practical and efficient ways to maximize anaerobic culture of Clostridium perfringens on blood agar date applies the... 800-443-3108 Fax: 408-762-7990 Email: customerservice @ anaerobesystems.com, Staphylococcus aureus or Enterococcus faecalis, Clostridium difficile Propionibacterium..., dass die … agar Clostridium perfringens on blood agar package until used anaerobic... Different-Looking colony types, so the culture appears mixed woman with including gamma- and delta-toxin, native! Bacilli ( rod ) staining gram-positive red component of the tail of a test mouse the of... Time I comment by CLSI for quality assurance performance testing at Anaerobe Systems Organisms specified by clostridium perfringens hemolysis blood agar for assurance! A sheep and hemolyze sheep blood agar base ( BBL ), Mobiluncus Actinomyces... 408-762-7990 Email: customerservice @ anaerobesystems.com, Staphylococcus aureus or Enterococcus faecalis, Clostridium or... 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